The analysis of the stool for worms and worm eggs - directions for research

Currently, the analysis of the stool for worms is routine laboratory and diagnostic manipulation, to assign to, the children and the adults, as in the course of the investigation with the objective of identifying the causes of pathological symptoms, and in the course of preventive annual medical examination. This analysis, despite the spread, not always understandable to the people, to consider his appointment, the methodology for the conduct and transcription of results.

Research on enterobiasis

The analysis of the stool for worms (worm eggs) – common feature

The analysis of the feces for the worms properly is called the analysis on eggs a worm, and since in the course of execution of the manipulation and dependent diagnostic laboratory explores the stool to verify the presence of eggs of various parasitic worms which may affect organs and tissues of the human body. And as the collective name of all the parasitic worms is worms, the main objective of the analysis is the identification or confirmation of the lack of eggs data of parasites.

The fact is that in various organs and tissues of the human body can parasitize about 250 types of worms, which reproduce to lay eggs. These eggs within the intestines and come out together with faeces. The study smear the feces under the microscope you can see with the naked eye the larvae and the eggs, variety of parasitic worms, then, to diagnose their presence. That is, the analysis of feces for worm eggs is a method of diagnosis of helminthic diseases, caused by worms (worms).

Any parasites (worms) it is possible to identify through the analysis of feces?

In the course applied in the time of laboratory methodologies for the analysis of feces for worm eggs may reveal the presence of the following parasitic worms (helminths):

Class nematode (nematodes):

  • Roundworms (Ascaris lumbricoides);
  • Trichuris trichuris (Trichocephalus trichiurus);
  • Thominx aerofilus;
  • Ancylostoma duodenal;
  • Necator (Necator americanus);
  • Strongyloides stercoralis.

Class of flatworms (flukes-flukes):

  • Plaice liver (Fasciola hepatica);
  • Pussy cats (Opisthorchis felineus);
  • Dicrocoelium lanceatum;
  • Schistosoma mansoni et japonicum.

Class tapeworms (cestody):

  • The tapeworm unarmed (Taeniarhynchus saginatus);
  • The tapeworm armed (Taenia solium);
  • A tapeworm (Diphyllobothrium latum);
  • Tapeworm small (Diphyllobothrium minus).
Diagnostics

That is, all of these types of parasites worms can be identified in the course of the usual analysis of feces for worm eggs. You should understand that in the faeces was not found if the same helminths, and the particles of their skins, or eggs, repressed for playback. Therefore, if the doctor sees in the feces the eggs of any parasites worms, then that means postpone their could only the helminths and, therefore, has in some way impressed in the body of that particular person. That is, in the presence of eggs of worms in the stool can be 100 % certainty say that a person is infected the data by a worm parasite, which is necessary to bring the body.

In addition to these worms for the analysis of feces for worm eggs, it is possible to identify the infection that are simple, such as, for example:

  • Entamoeba histolytica (Entamoebahistolytica);
  • Giardia (Giardialamblia).

Also in calais can be detected, cyclosporine and their larvae.

When we do the analysis of the feces for worm eggs (directions for research)

The analysis of the feces for worm eggs and surrendered in two possible cases:

Suspect helminths (intestinal worms);

Barrier analysis.

Barrier analysis is mandatory for the research, engaged in routine work, the procedure of investigation of people, coming for the work, in connection with the sale, processing or storage of food, contacts with local people and other sectors of activity, in which it is possible to transfer of helminths of the other. In this situation, the analysis of feces for worm eggs is a screen -, that is, used for the mass and rapid diagnosis helminths many people. Briefly we can say that as a required barrier for the analysis of feces for worm eggs surrenders to the registration of the health portable for admission to kindergarten, school, swimming pool or before a scheduled hospital admission. This analysis is necessary to deliver, because the man will be in contact with others, and if it is a train of worms, can infect others, thus extending the disease.

The second indication for the analysis of feces for worm eggs is suspected of any of the helminths. The suspect transmitted (infestation of maggots) occurs in the presence of different people with variable symptoms of disorders of the functioning of the various organs in the absence of disease. The fact is that the parasitic worms (helminths) can affect different organs and tissues, for example, the intestine, the bladder, the urethra, the brain, the liver, the heart, lymph nodes, and other, resulting in symptoms of various diseases. If the survey is not detected the disease of a body from which are the pathological symptoms occurs legitimate suspicion of a bot that requires a confirmation or denial. With the aim to confirm or refute helminthiasis surrenders the analysis of feces for worm eggs in such a situation.

Preparation for examination for analysis of the stool for worms

The analysis of the feces for worm eggs suitable for the delivery, in the presence of a person with the following symptoms:

  • Inflammatory diseases of the gastrointestinal tract (e.g., stomach pain, diarrhea, constipation, flatulence, etc.);
  • Fatigue;
  • Irritability;
  • Dream haunting;
  • Bruxism (creak the teeth);
  • Itching in the anal area;
  • Infectious in women;
  • Infection of the urinary tract;
  • Delay in growth and mass of a body that grows with children and adolescents;
  • Increase in the number of eosinophils in the blood;
  • The allergic syndrome dark of nature, in which it is not possible to identify the allergen, the caller pathological response on the part of the body;
  • Often colds and viral infections.

In the presence of these symptoms, it is necessary to do a stool examination for worm eggs, because they are sufficient factors of suspicion on the bot. However, only the analysis of the stool may be sufficient for diagnosis helminthiasis, and if you suspect parasites will have to take the tests, bile, phlegm, of the contents of the duodenum and other biological secrets of the human body, which can be the eggs or larvae of parasitic worms.

Preparation for examination for analysis of the stool for worms

Before giving in drops on worm eggs should be within a week do not take castor oil or mineral oil, antibiotics, anti-diarrheal and pesticides, and drugs also of bismuth and magnesium. Also for 7 – 14 days prior to the delivery cala on worm eggs is not recommended irrigoscopy.

In addition, you must psychologically and morally in tune with what is, for the quality diagnostic helminths in need of one, three or six samples of feces, which will need to be taken every day, or every 1 – 2 days. Usually need three stool samples, but if there is a suspicion of dysentery amoeba or giardia, well exceed the six stool samples.

It is recommended that you prepare in advance dishes for stool samples. Are not currently used and familiar to many people with children, boxes of matches, for the laboratory to give special plastic containers with cover. If in the laboratory, the container was not given, it is possible to buy normal drugstore. Better to use exactly the type of disposable container, and not any existing home dishes or a box, because there may be larvae or eggs of parasites, placed her hands dirty, which in the study were identified and, then, a person will get a false-positive result.

The laboratory

No special preparation of the examination for the analysis of feces for worm eggs to produce is not necessary.

How to properly assemble the stool for analysis of worm eggs?

Before the faeces need to urinate, the urine has to faecal bulk. So it is necessary, it is not my skin the anal, you poop into a receptacle, the position will be to recruit the stool samples (for example, a pot, a basin, etc.).

From the whole mass of stool, you will need to collect a small amount corresponding to about two teaspoons, by removing the trials with different pieces of feces special magic wand. If the container for the faeces of no, then it is possible to collect samples of the routine with a match or a stick. Ideally take 3 – 4 small pieces for the sides, the front and rear parts of the upper surface, and also within the fecal matter. All the recovered part of cala placed in a tight container and close the lid. The container is glued to a piece of paper with the surname, name and patronymic, and quickly transported to the laboratory.

For more accurate results in the optimal way to deliver the samples of stool for the worm eggs in the laboratory for 30 – 45 minutes after defecation, and their fence. However, if for a short period of time to deliver a stool sample in the laboratory impossible, you can do so within a maximum of 5 – 8 hours, necessarily keeping the stool in the refrigerator at a temperature of +4 +8, orC. If the samples of your stool for more than 60 minutes is left in the fridge, in a warm environment, thus are not suitable to put on worm eggs. In this case, it is necessary to throw away a rotten test, and collect a new one after defecation.

For a precise and complete diagnosis, it is necessary to pass three stool samples. In addition, the collection of samples of all the days, or after 1 – 2 days. The final result of the research can only be obtained after a search of three tests.

The result of the analysis of the feces for worm eggs and its transcription

In accordance with the result of an analysis of stool for worm eggs in all three tests must be negative. This means that the eggs of helminths were not detected, and the man was not infected from the worms.

A positive result in at least one of the fecal samples is an indication that a man has found helminths, and, as a result, has been infected from the worms. In such a situation, it is necessary to undergo a treatment and one month after the end of support again, the analysis of feces.

If the result of the analysis of the feces for worm eggs positive, indicates what type of parasites helminths has been discovered. Example of a positive formulation for the analysis of feces for worm eggs is the following: "Positive, the eggs of ascaris". In each laboratory formulation may vary slightly, but will not necessarily be present, the term "positive", indicating the presence of worm parasites, and the name of the species of worms, the eggs of which were detected.

What are the methods use in the laboratory for the analysis of feces for worm eggs?

The identification of parasites worms in stool samples in the laboratory produces with methods moprovskaja (helminthologia), that is, the detection of eggs of helminths. In the laboratories for the diagnosis of parasites in humans typically use three main methods moprovskaja :

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  1. Microscopy-native smear;
  2. The Method Fulleborn;
  3. The Method Of Telemann.

Usually the test of cala constantly subjected to all three methods moprovskaja . In the first place, produce microscopy-native smear, and then randomly make a test Fulleborn and Telemann.

Microscopy-native smear

Microscopy-native smear is careful examining under the microscope a small drop of feces, smeared a thin layer on a glass plate. For this, the glass is first applied a drop of 50% glycerol, which facilitates the uniform distribution of the material. Then take a small piece of feces and mixed with distilled water up to a state of sour cream. Finished mass cala take a small drop, placed on the glass, and stir with a wooden stick with glycerin. Then this same wand drop to the smear on a glass surface with a thin layer. After that, the above smear to put a glass cover, and consider the material under the microscope.

The Test Fulleborn

The test Fulleborn consists of a mixture of feces with salt water, which has a high density and specific weight, because of this that the eggs of helminths will float on its surface. To perform a test of 10 g of cala mix with 100 ml 50% saturated sodium chloride solution. The solution was prepared in the following way: – 450 – 500 g of salt dissolved in 1 liter of water, the heat up temperature of 50 – 70oC.

Mixture of cala salt water put in a quiet place in 1 – 1.5 hours for the eggs of helminths revealed the solution. After the necessary amount of time that it appears on the surface of the film to alleviate the cycle, and apply onto a slide. The film, close the cover glass and under a microscope.

The Proof Of Telemann

Analysis

The proof of the Telemann is a mixture of cala with hydrochloric acid and the ether, which show that the eggs of the worms. For the production of samples of the Telemann a small amount of feces mixed with 3 – 4 ml 30% hydrochloric acid and 15 – 20 ml of distilled water. Then the fog cala add 1 – 1.5 ml of ether, mix well and close the jar tightly ground glass or the rubber cover. Then the ship several times to tilt the neck the top to the bottom, stirring the contents. After this the suspension is filtered through a layer of gauze and centrifuged. Obtained after centrifugation the pellet is put on a slide, close the cover glass and under a microscope.

If at least one test were detected eggs of helminths, the result of the analysis is considered to be positive.

The analysis of the stool for worms – where to rent?

Do a stool examination for worms can be in any of the clinical-diagnostic laboratory in a clinic or hospital. However, for the maximum reliability of the result it is recommended that you take a stool sample specialized bacteriological laboratories, which are available when the sanitary-epidemiological services of each city or district.

In addition, to deliver analysis in a private lab or clinic, which has the licence for the performance of such a diagnostic search.

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01.06.2019